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HEK293T cells were cotransfected with pCF-BBtr, the empty vector pcDNA, or pcfeA3Z2b-HA, and increasing amounts of plasmids encoding either wt or mutant Bet (Figure。

FeFAB cells (a kind gift from Dr Martin Löchelt, Germany) are modified CRFK cells that are used for FFV infection experiments. FeFAB cells express β-galactosidase under the control of the FFV LTR, which is trans。

FeFAB assay indicated that the culture supernatants collected from the infected and drug-pretreated cells contained infectious FFV virions, which induce expression of the β-galactosidase infefab the ...

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